Document Type
Article
Publication Date
2-2011
Journal or Book Title
Insect Molecular Biology
Volume
20
Issue
1
First Page
15
Last Page
27
DOI
10.1111/j.1365-2583.2010.01046.x
Abstract
The movement of miniature inverted repeat transposable elements (MITEs) modifies genome structure and function. We describe the microsatellite-associated interspersed nuclear element 2 (MINE-2), that integrates at consensus WTTTT target sites, creates dinucleotide TT target site duplications (TSDs), and forms predicted MITE-like secondary structures; a 5' subterminal inverted repeat (SIR; AGGGTTCCGTAG) that is partially complementary to a 5' inverted repeat (IR; ACGAAGCCCT) and 3'-SIRs (TTACGGAACCCT). A (GTCY)(n) microsatellite is hitchhiking downstream of conserved 5'MINE-2 secondary structures, causing flanking sequence similarity amongst mobile microsatellite loci. Transfection of insect cell lines indicates that MITE-like secondary structures are sufficient to mediate genome integration, and provides insight into the transposition mechanism used by MINE-2s.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Coates, Brad S.; Kroemer, J. A.; Sumerford, Douglas V.; and Hellmich, Richard L., "A novel class of miniature inverted repeat transposable elements (MITEs) that contain hitchhiking (GTCY)n microsatellites" (2011). Entomology Publications. 135.
https://lib.dr.iastate.edu/ent_pubs/135
Comments
This article is from Insect Molecular Biology; 20 (2011); 15-27; doi: 10.1111/j.1365-2583.2010.01046.x