Document Type
Article
Publication Date
2009
Journal or Book Title
The American journal of tropical medicine and hygiene
Volume
80
Issue
1
First Page
85
Last Page
95
Abstract
As part of our ongoing surveillance efforts for West Nile virus (WNV) in the Yucatan Peninsula of Mexico, 96,687 mosquitoes collected from January through December 2007 were assayed by virus isolation in mammalian cells. Three mosquito pools caused cytopathic effect. Two isolates were orthobunyaviruses (Cache Valley virus and Kairi virus) and the identity of the third infectious agent was not determined. A subset of mosquitoes was also tested by reverse transcription-polymerase chain reaction (RT-PCR) using WNV-, flavivirus-, alphavirus-, and orthobunyavirus-specific primers. A total of 7,009 Culex quinquefasciatus in 210 pools were analyzed. Flavivirus RNA was detected in 146 (70%) pools, and all PCR products were sequenced. The nucleotide sequence of one PCR product was most closely related (71-73% identity) with homologous regions of several other flaviviruses, including WNV, St. Louis encephalitis virus, and Ilheus virus. These data suggest that a novel flavivirus (tentatively named T'Ho virus) is present in Mexico. The other 145 PCR products correspond to Culex flavivirus, an insect-specific flavivirus first isolated in Japan in 2003. Culex flavivirus was isolated in mosquito cells from approximately one in four homogenates tested. The genomic sequence of one isolate was determined. Surprisingly, heterogeneous sequences were identified at the distal end of the 5' untranslated region.
Copyright Owner
Jose A. Farfan-Ale, et al
Copyright Date
2009
Language
en
File Format
application/pdf
Recommended Citation
Farfan-Ale, Jose A.; Lorono-Pino, Maria A.; Garcia-Rejon, Julian E.; Hovav, Einat; Powers, Ann M.; Lin, Ming; Dorman, Karin S.; Platt, Kenneth B.; Bartholomay, Lyric; Soto, Victor; Beaty, Barry J.; Lanciotti, Robert S.; and Blitvich, Bradley J., "Detection of RNA from a Novel West Nile-like Virus and High Prevalence of an Insect-specific Flavivirus in Mosquitoes in the Yucatan Peninsula of Mexico" (2009). Entomology Publications. 154.
https://lib.dr.iastate.edu/ent_pubs/154
Included in
Entomology Commons, Molecular Genetics Commons, Virology Commons, Virus Diseases Commons
Comments
This is an author's manuscript of an article from The American journal of tropical medicine and hygiene 80 (2008): 85.