Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus

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2008-08-23
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Li, Huarong
Sparks, Wendy
Bonning, Bryony
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Bonning, Bryony
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Entomology
Abstract

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran Drosophila Development and Reproduction, a JoVE Science Education video explaining more about about the context of larvae" >larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.

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This article is from Journal of Visual Experiments 18 (2008): doi:10.3791/889. Posted with permission.

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Tue Jan 01 00:00:00 UTC 2008
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