Campus Units
Entomology, Biomedical Sciences
Document Type
Article
Publication Version
Published Version
Publication Date
8-2015
Journal or Book Title
Insect Biochemistry and Molecular Biology
Volume
63
First Page
47
Last Page
53
DOI
10.1016/j.ibmb.2015.04.008
Abstract
The southern cattle tick (Rhipicephalus (Boophilus) microplus) is a hematophagous external parasite that vectors the causative agents of bovine babesiosis or cattle tick fever, Babesia bovis and B. bigemina, and anaplasmosis, Anaplasma marginale. The southern cattle tick is a threat to the livestock industry in many locations throughout the world. Control methods include the use of chemical acaricides including amitraz, a formamidine insecticide, which is proposed to activate octopamine receptors. Previous studies have identified a putative octopamine receptor from the southern cattle tick in Australia and the Americas. Furthermore, this putative octopamine receptor could play a role in acaricide resistance to amitraz. Recently, sequence data indicated that this putative octopamine receptor is probably a type-1 tyramine receptor (TAR1). In this study, the putative TAR1 was heterologously expressed in Chinese hamster ovary (CHO-K1) cells, and the expressed receptor resulted in a 39-fold higher potency for tyramine compared to octopamine. Furthermore, the expressed receptor was strongly antagonized by yohimbine and cyproheptadine, and mildly antagonized by mianserin and phentolamine. Tolazoline and naphazoline had agonistic or modulatory activity against the expressed receptor, as did the amitraz metabolite, BTS-27271; however, this was only observed in the presence of tyramine. The southern cattle tick's tyramine receptor may serve as a target for the development of anti-parasitic compounds, in addition to being a likely target of formamidine insecticides.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Gross, Aaron D.; Temeyer, Kevin G.; Day, Timothy A.; Pérez de León, Adalberto A.; Kimber, Michael J.; and Coats, Joel R., "Pharmacological Characterization of a Tyramine Receptor from the Southern Cattle Tick, Rhipicephalus (Boophilus) microplus" (2015). Entomology Publications. 302.
https://lib.dr.iastate.edu/ent_pubs/302
Comments
This article is from Insect Biochemistry and Molecular Biology 63 (2015): 47, doi:10.1016/j.ibmb.2015.04.008.