Campus Units

Entomology

Document Type

Article

Publication Version

Published Version

Publication Date

2016

Journal or Book Title

Journal of Agricultural and Food Chemistry

Volume

64

Issue

11

First Page

2175

Last Page

2189

DOI

10.1021/acs.jafc.5b03766

Abstract

The widespread use of Cry proteins in insecticide formulations and transgenic crops for insect control has led to an increased interest in the environmental fate of these proteins. Although several detection methods are available to monitor the fate of Cry proteins in the environment, enzyme-linked immunosorbent assays (ELISAs) have emerged as the preferred detection method, due to their cost-effectiveness, ease of use, and rapid results. Validation of ELISAs is necessary to ensure accurate measurements of Cry protein concentrations in the environment. Validation methodology has been extensively researched and published for the areas of sensitivity, specificity, accuracy, and precision; however, cross validation of ELISA results has been studied to a lesser extent. This review discusses the use of ELISAs for detection of Cry proteins in environmental samples and validation of ELISAs and introduces cross validation. The state of Cry protein environmental fate research is considered through a critical review of published literature to identify areas where the use of validation protocols can be improved.

Comments

This article is published as Albright III, Vurtice C., Richard L. Hellmich, and Joel R. Coats. "A review of Cry protein detection with enzyme-linked immunosorbent assays." Journal of agricultural and food chemistry 64, no. 11 (2016): 2175-2189. doi: 10.1021/acs.jafc.5b03766 .

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

Share

COinS