Campus Units

Entomology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

11-1999

Journal or Book Title

Medical and Veterinary Entomology

Volume

13

Issue

4

First Page

377

Last Page

385

DOI

10.1046/j.1365-2915.1999.00185.x

Abstract

To study the population structure of Glossina morsitans morsitans Westwood (Diptera: Glossinidae), polymerase chain reaction (PCR) and single‐strand conformational polymorphism (SSCP) methods were used to estimate mitochondrial DNA diversity at four loci in six natural populations from Zambia, Zimbabwe and Mozambique, and in two laboratory cultures. The Zambian and Zimbabwean samples were from a single fly belt. Four alleles were recorded at 12S and 16S1, and five alleles at 16S2 and COI. Nucleotide sequencing confirmed their singularities. Chi‐square contingency tests showed that allele frequencies differed significantly among populations. Mean allele diversities in populations averaged over loci varied from 0.14 to 0.61. Little loss in haplotype diversity was detected in the laboratory cultures thereby indicating little inbreeding. Wright’s fixation index FST in the natural populations was 0.088 ± 0.016, the correlation of haplotypes within populations relative to correlations in the total. A function of its inverse allows an estimate of the mean equivalent number of females exchanged per population per generation, 5.2. No correlation was detected between pairwise genetic distance measures and geographical distances. Drift explains the high degree of differentiation.

Comments

This is the peer reviewed version of the following article: Wohlford, D. L., E. S. Krafsur, N. T. Griffiths, J. G. Marquez, and M. D. Baker. "Genetic differentiation of some Glossina morsitans morsitans populations." Medical and veterinary entomology 13, no. 4 (1999): 377-385, which has been published in final form at doi: 10.1046/j.1365-2915.1999.00185.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Copyright Owner

Blackwell Science Ltd

Language

en

File Format

application/pdf

Published Version

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