Campus Units

Entomology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

3-2006

Journal or Book Title

Medical and Veterinary Entomology

Volume

20

Issue

1

First Page

138

Last Page

149

DOI

10.1111/j.1365-2915.2006.00609.x

Abstract

The origins of extant G. pallidipes Austen (Diptera: Glossinidae) populations in the ecologically well studied Lambwe and Nguruman valleys in Kenya are controversial because populations have recovered after seemingly effective attempts to achieve high levels of control. We investigated microgeographic breeding structure of the tsetse fly, Glossina pallidipes (Diptera: Glossinidae) by analyzing spatial and temporal variation at eight microsatellite loci to test hypotheses about endemism and immigration. Samples were obtained at seasonal intervals from trap sites separated by 200 m to 14 km and arranged into blocks. G. pallidipes populations nearest to Lambwe and Nguruman also were sampled. Spatial analysis indicated genetic differentiation by genetic drift was much less among trapping sites within Lambwe and Nguruman (FST ≤ 0.049) than between them (FST = 0.232). FST between Serengeti and Nguruman was 0.16 and FST between Kodera Forest and Lambwe was 0.15. The genetic variance in G. pallidipes explained by dry and wet seasons (0.33%) was about one-fifth the variance among collection dates (1.6%) thereby indicating reasonable temporal stability of genetic variation. Gene frequencies in Kodera and Serengeti differed greatly from Lambwe and Nguruman thereby falsifying the hypothesis that Lambwe and Nguruman were repopulated by immigrants. Harmonic mean effective (= breeding) population sizes were 180 in Lambwe and 551 in Nguruman. The genetic data suggest G. pallidipes in Lambwe and Nguruman have been endemic for long intervals.

Comments

This is the peer reviewed version of the following article: Ouma, J. O., J. G. Marquez, and E. S. Krafsur. "Microgeographical breeding structure of the tsetse fly, Glossina pallidipes in south‐western Kenya." Medical and veterinary entomology 20, no. 1 (2006): 138-149, which has been published in final form at doi: 10.1111/j.1365-2915.2006.00609.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

Copyright Owner

The Authors; Journal compilation The Royal Entomological Society

Language

en

File Format

application/pdf

Published Version

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