New Polymorphic Microsatellites in Glossina pallidipes (Diptera: Glossinidae) and Their Cross-Amplification in Other Tsetse Fly Taxa

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2006-10-01
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Ouma, J. O.
Marquez, J. G.
Krafsur, E. S.
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Krafsur, Elliot
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Entomology

The Department of Entomology seeks to teach the study of insects, their life-cycles, and the practicalities in dealing with them, for use in the fields of business, industry, education, and public health. The study of entomology can be applied towards evolution and ecological sciences, and insects’ relationships with other organisms & humans, or towards an agricultural or horticultural focus, focusing more on pest-control and management.

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The Department of Entomology was founded in 1975 as a result of the division of the Department of Zoology and Entomology.

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We report the development and characterization of three new microsatellite markers in the tsetse fly, Glossina pallidipes (Diptera: Glossinidae). Fifty-eight alleles were scored in 192 individuals representing six natural populations. Allelic diversity ranged from 9 to 28 alleles per locus (mean 19.3 ± 5.5). Averaged across loci, observed heterozygosity was 0.581 ± 0.209, and expected heterozygosity was 0.619 ± 0.181. Cross-species amplifications of the G. pallidipesloci in other tsetse fly taxa are reported.

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This is a manuscript of an article published as Ouma, J. O., J. G. Marquez, and E. S. Krafsur. "New polymorphic microsatellites in Glossina pallidipes (Diptera: Glossinidae) and their cross-amplification in other tsetse fly taxa." Biochemical genetics 44, no. 9-10 (2006): 464-470.. doi: 10.1007/s10528-006-9046-x. Posted with permission.

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Sun Jan 01 00:00:00 UTC 2006
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