New Polymorphic Microsatellites in Glossina pallidipes (Diptera: Glossinidae) and Their Cross-Amplification in Other Tsetse Fly Taxa
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Abstract
We report the development and characterization of three new microsatellite markers in the tsetse fly, Glossina pallidipes (Diptera: Glossinidae). Fifty-eight alleles were scored in 192 individuals representing six natural populations. Allelic diversity ranged from 9 to 28 alleles per locus (mean 19.3 ± 5.5). Averaged across loci, observed heterozygosity was 0.581 ± 0.209, and expected heterozygosity was 0.619 ± 0.181. Cross-species amplifications of the G. pallidipesloci in other tsetse fly taxa are reported.
Comments
This is a manuscript of an article published as Ouma, J. O., J. G. Marquez, and E. S. Krafsur. "New polymorphic microsatellites in Glossina pallidipes (Diptera: Glossinidae) and their cross-amplification in other tsetse fly taxa." Biochemical genetics 44, no. 9-10 (2006): 464-470.. doi: 10.1007/s10528-006-9046-x. Posted with permission.