Document Type

Article

Publication Date

2002

Journal or Book Title

Current Genetics

Volume

41

Issue

6

First Page

414

Last Page

424

DOI

10.1007/s00294-002-0317-8

Abstract

An optional group I intron was characterized at a single insertion point in nuclear small subunit rRNA (nuSSU rRNA) genes of the imperfect entomopathogenic fungi, Beauveria bassiana and B. brongniartii. Insertion points were conserved among nuSSU rRNA genes from 35 Beauveria isolates. PCR-RFLP and DNA sequencing identified 12 group I intron variants and were applied to the identification of strains isolated from insect hosts. Alignment of 383– 404-nt subgroup IB3 group I introns indicated that four insertion/deletion (indel) mutations were the main basis of fragment length variation. Phylogeny reconstruction using parsimony and neighbor-joining methods suggested six lineages may be present among nuSSU rRNA group I intron sequences from Beauveria and related ascomycete fungi. Terminal node placement of Beauveria introns conflicted with previously published phylogenies constructed from gene sequences, suggesting horizontal transfer of group I introns. PCRRFLP among introns provided a means for the differentiation of Beauveria isolates.

Comments

This article is from Current Genetics; 41 (2002); 414-424; doi: 10.1007/s00294-002-0317-8

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Copyright Owner

Springer-Verlag

Language

en

File Format

application/pdf

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