Degree Type
Dissertation
Date of Award
2011
Degree Name
Doctor of Philosophy
Department
Chemical and Biological Engineering
First Advisor
Aaron R. Clapp
Abstract
Quantum dots (QDs) are considered as fantastic substitutes for organic dyes or genetically coded proteins because of their outstanding optical properties including broad absorption spectra, size tunable emission spectra, large extinction coefficient and superior resistance to chemical and physical degradation. They are now widely used in many biological applications. However, QDs are generally synthesized using organometallic approach and only soluble in organic solvents. To transfer QDs into aqueous solution, we replaced their native ligands with a new group of water-soluble molecules based on simple yet flexible dithiocarbamate chemistry. This cap exchange method offers QDs not only solubility but also stability. We then investigate their applications in cellular labeling and \textit{in vitro} assay by making use of their unique optical properties.
To extend QDs application, we encapsulate them into amphiphilic polymers using micromixers (confined impinging jets reactor (CIJR) and multi-inlets vortex mixer (MIVM)). They are much brighter than single QD and they can be potentially used in optical barcoding. We are able to generate particles in a wide range of size from 90nm to 800nm by exploiting single mixer or mixers in series.
Copyright Owner
Yanjie Zhang
Copyright Date
2011
Language
en
Date Available
2012-04-06
File Format
application/pdf
File Size
194 pages
Recommended Citation
Zhang, Yanjie, "Surface functionalization of quantum dots for biotechnological applications" (2011). Graduate Theses and Dissertations. 10266.
https://lib.dr.iastate.edu/etd/10266
