Degree Type

Thesis

Date of Award

2009

Degree Name

Master of Science

Department

Biochemistry, Biophysics and Molecular Biology

First Advisor

Basil J. Nikolau

Abstract

Here we describe the identification and characterization of the Arabidopsis homolog (At2g01170.1) to the S. cerevisiae BIO5 transporter of the biotin precursor 7-keto 8-aminopelargonic acid (KAPA). The Arabidopsis protein encoded by At2g01170 (also called BAT1) shares 22.1% identity and 36.8% similarity to the S. cerevisiae BIO5 protein, and the two proteins have highly conserved computationally predicted secondary structures, with both proteins predicted to have 12 α-helical trans-membrane domains. The Arabidopsis BIO5 homolog was also able to complement S. cerevisiae bio5 mutants. Arabidopsis At2g01170 (bio5) mutants did not have the growth phenotype observed in S. cerevisiae bio5 mutants though there was an impact on the biotin content associated with the mutant. The previously unknown subcellular location of BIO5 was determined to be in the plasma membrane by a GFP tagging study. This Arabidopsis BIO5 homolog is proposed to facilitate the distribution of biotin or its precursors, such as KAPA among the plant's cells and tissues.

DOI

https://doi.org/10.31274/etd-180810-2100

Copyright Owner

Lucas John Showman

Language

en

Date Available

2012-04-30

File Format

application/pdf

File Size

47 pages

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