Degree Type

Dissertation

Date of Award

2010

Degree Name

Doctor of Philosophy

Department

Chemistry

First Advisor

Emily A. Smith

Abstract

In order to better understand the cell signaling process, non-invasive methods to measure the integrin microclustering and examine the factors affecting the dynamics of these clusters are needed. This dissertation is focused on the development of non-invasive analytical tools to investigate the role of cytoplasmic proteins and cholesterol on the nanoscale dynamic localization of integrins using a technique called FRET, fluorescence resonance energy transfer. FRET is used to detect the proximity of molecules at nanometer distances (1-10 nm) and can be applied in diverse ways with various microscope configurations. FRET images obtained with such setups can be used to quantify the interactions between biomolecules within cells.

DOI

https://doi.org/10.31274/etd-180810-2142

Copyright Owner

Deepak Dibya

Language

en

Date Available

2012-04-30

File Format

application/pdf

File Size

117 pages

Included in

Chemistry Commons

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