Degree Type

Dissertation

Date of Award

2010

Degree Name

Doctor of Philosophy

Department

Chemistry

First Advisor

Emily A. Smith

Abstract

In order to better understand the cell signaling process, non-invasive methods to measure the integrin microclustering and examine the factors affecting the dynamics of these clusters are needed. This dissertation is focused on the development of non-invasive analytical tools to investigate the role of cytoplasmic proteins and cholesterol on the nanoscale dynamic localization of integrins using a technique called FRET, fluorescence resonance energy transfer. FRET is used to detect the proximity of molecules at nanometer distances (1-10 nm) and can be applied in diverse ways with various microscope configurations. FRET images obtained with such setups can be used to quantify the interactions between biomolecules within cells.

Copyright Owner

Deepak Dibya

Language

en

Date Available

2012-04-30

File Format

application/pdf

File Size

117 pages

Included in

Chemistry Commons

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