Degree Type

Dissertation

Date of Award

2008

Degree Name

Doctor of Philosophy

Department

Chemistry

First Advisor

Robert S. Houk

Abstract

The development of mass spectrometry (MS) instrumentation for novel biological applications, specifically, the development of instrumentation that integrates ion/ion reaction capabilities in an ion trap (IT) with ion mobility-quadrupole-time-of-flight (IMS-q-TOF) analysis is presented. Chapter 1 provides a general introduction to protein analysis by MS, ion/ion reactions and ion/ion reaction instrumentation, and IMS techniques and IMS instrumentation. Chapter 2 describes the construction and performance of a linear ion trap (LIT) made with primarily commercially available components. The LIT has two ion sources for independent analyte ion and reagent ion formation. The LIT functions as a reaction vessel for gas-phase ion/ion reactions and as a mass spectrometer using mass-selective axial ejection. Initial experiments demonstrate the LIT's ability to perform both dual polarity storage mode and transmission mode proton transfer ion/ion reactions. Chapter 3 describes the construction and performance of an IT-IMS-q-TOF with three independent ion sources. This instrument is the first MS to combine ion/ion reaction capabilities with IMS-q-TOF analysis. Chapter 4 describes novel experiments performed on the IT-IMS-q-TOF instrument constructed in our lab. The gas phase conformation of cytochrome c ions in multiple different charge states is investigated using proton transfer ion/ion reactions and IMS-q-TOF analysis.

Copyright Owner

Matthew William Soyk

Language

en

Date Available

2012-04-30

File Format

application/pdf

File Size

127 pages

Included in

Chemistry Commons

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