Degree Type
Thesis
Date of Award
2010
Degree Name
Master of Science
Department
Food Science and Human Nutrition
First Advisor
Ruth S. Macdonald
Abstract
The incidence of inflammatory bowel diseases (IBD) increased recently. Within the colon, the family of mucins and trefoil factors (TFF) facilitate mucosal protection. Our objective was to determine if diet and/or administration of LGG would attenuate DSS-induced colitis by altering expression of the MUC and TFF genes. C57BL/6 mice were fed diets containing casein, soy, or whey protein with or without LGG for 12 days. Seven days after LGG, the mice were given 2% DSS for 4 days. One day after DSS treatment the colonic and cecal tissues and fecal samples were collected and analyzed by quantitative RT-PCR. Whey protein significantly increased cecal LGG content. MUC1 expression was induced by DSS and soy protein dramatically suppressed it. TFF3 was upregulated by DSS and decreased by soy and whey protein. Soy protein also reduced the inflammatory scores, TNF-α and prevented colon shortening. LGG did not have effect on these parameters. In conclusion, soy protein suppressed the DSS-induced inflammatory stimulation of MUC, TFF and TNF-α gene expression. Whey protein may act as prebiotic, but failed to prevent colitis.
DOI
https://doi.org/10.31274/etd-180810-1957
Copyright Owner
Huanyi Jiang
Copyright Date
2010
Language
en
Date Available
2012-04-30
File Format
application/pdf
File Size
105 pages
Recommended Citation
Jiang, Huanyi, "Reduction in Mucosal Barrier Markers with Soy Protein Diet but not Lactobacillus rhamnosus GG in DSS-Treated Mice" (2010). Graduate Theses and Dissertations. 11805.
https://lib.dr.iastate.edu/etd/11805
