Degree Type

Dissertation

Date of Award

2011

Degree Name

Doctor of Philosophy

Department

Agronomy

First Advisor

Linda M. Pollak

Second Advisor

A. Susana Goggi

Abstract

The steady increase of genetically-modified (GM) corn (Zea mays L.) in the U.S. makes production of transgene-free corn more difficult to achieve. A strategy to minimize cross-contamination of non-GM corn with GM corn is by using unilateral cross-incompatibility controlled by the Ga1S allele. This allele is transferred to desired germplasm through backcrossing before cross-incompatible Ga1S/Ga1S lines are obtained. The usefulness of this system in preventing cross-fertilization with foreign ga1 pollen might be affected by a combination of environmental effects and heritability of the trait. Methods used for selection of cross-incompatibility in maize are based on differential incompatibility of plants to ga1 pollen. Chapter 2 investigates the efficiency of SSR molecular markers in predicting zygosity at the ga1 locus compared with conventional phenotyping. Chapter 3 evaluates the effect of genotype, environment, and genotype x environment effects on cross-incompatibility, and estimate its heritability.

Copyright Owner

Moises David Gonzalez

Language

en

Date Available

2012-04-30

File Format

application/pdf

File Size

69 pages

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