Degree Type

Dissertation

Date of Award

2012

Degree Name

Doctor of Philosophy

Department

Veterinary Microbiology and Preventive Medicine

First Advisor

James A. Roth

Abstract

Three bovine monoclonal antibodies (MAb) which neutralized bovine herpesvirus 1 (BHV1) were generated using a unique `self-refusion' method. Each was determined to be directed to one of the three major neutralization targets of BHV1 (gB, gC, and gD), and was capable of passive immunization in a laboratory rabbit intravenous challenge model. They were found to be specific for the immunizing BHV1 or for BHV1 and the closely related BHV5. The epitope specificities of two were identified using characterized murine MAb. The previously unidentified combination of epitope and virus reactivity of the two bovine MAb suggest a sequence-structural difference in gB between BHV1.1 and BHV5, and an epitope adjacent to a known epitope for the anti-gD bovine MAb. Anti-idiotypic antibodies were generated against one of the bovine MAb for possible use in vaccination. Bovine MAb may offer unique advantages for research and application in diagnostics, vaccine development, and in better understanding the bovine immune response to infectious disease.

Copyright Owner

Randall L. Levings

Language

en

File Format

application/pdf

File Size

92 pages

Share

COinS