Degree Type

Dissertation

Date of Award

2012

Degree Name

Doctor of Philosophy

Department

Chemistry

First Advisor

Robert S. Houk

Abstract

A variety of hyphenated mass spectrometry techniques were implemented for the analysis of biological compounds. Ion mobility combined with mass spectrometry was used to measure gas-phase conformations of proteins sprayed from slightly alkaline solutions. High-resolution mass spectrometry (HRMS) was utilized for a comprehensive analysis of alpha-zein proteins extracted from corn gluten meal (CGM) and distillers' dried grains with solubles (DDGS). Electrospray ionization (ESI) and tandem mass spectrometry were used to characterize phosphopeptides from the egg yolk protein phosvitin. And finally, a novel atmospheric pressure ionization source which combined UV laser desorption and atmospheric pressure photoionization was introduced.

Copyright Owner

Derrick Morast

Language

en

File Format

application/pdf

File Size

148 pages

Share

COinS