Degree Type

Thesis

Date of Award

2012

Degree Name

Master of Science

Department

Veterinary Microbiology and Preventive Medicine

First Advisor

Ronald W. Griffith

Abstract

Two commercial molecular assays, the PremiTest Salmonella and the xMAP Salmonella, were recently developed with the goal of identifying many of the most common Salmonella enterica serovars in less than eight hours. This study compared the results of these two assays to that of traditional serotyping on 233 isolates representing more than 52 different serovars, including the ten most frequently identified at the NVSL from 2000 to 2010. The PremiTest assay completely and correctly identified 150 isolates (64%) and the xMAP assay identified 181 isolates (78%). Both molecular assays were able to partially identify additional serovars. These assays offer a new, rapid approach to screening Salmonella isolates in the laboratory.

Copyright Owner

Brenda Renee Morningstar-Shaw

Language

en

File Format

application/pdf

File Size

56 pages

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