Degree Type

Thesis

Date of Award

2012

Degree Name

Master of Science

Department

Veterinary Microbiology and Preventive Medicine

First Advisor

Erin L. Strait

Abstract

Swine mycoplasmas are acknowledged for their potential to contribute to several swine disease complexes. In addition, mycoplasma-associated arthritis has recently been recognized as a re-emergent issue in the swine industry. Reports from Veterinary Diagnostic Laboratories suggest an increasing incidence of cases occurring in the major regions of swine production in the U.S. Although previous studies reproduced polyarthritis by inoculating pigs with either Mycoplasma hyosynoviae or M. hyorhinis, there is a lack of information regarding the epidemiology of these microorganisms within swine populations. In addition, although new diagnostic tools have become available, information regarding the application and performance of these assays is lacking.

Therefore, a major objective of the work described in this thesis was to review and to provide new information regarding the use of diagnostic methods in the detection of M. hyosynoviae and M. hyorhinis. In order to evaluate the performance of a new real-time polymerase chain reaction (RT-PCR), a comparison of various DNA extraction methods using different clinical specimens was conducted in the laboratory. Thereafter, a field study was carried using RT-PCR- and antibody-based assays to monitor the infection dynamics of these microorganisms in a wean-to-finish population.

DOI

https://doi.org/10.31274/etd-180810-253

Copyright Owner

Joao Carlos Gomes Neto

Language

en

File Format

application/pdf

File Size

59 pages

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