Date of Award
Master of Science
Veterinary Microbiology and Preventive Medicine
Erin L. Strait
Swine mycoplasmas are acknowledged for their potential to contribute to several swine disease complexes. In addition, mycoplasma-associated arthritis has recently been recognized as a re-emergent issue in the swine industry. Reports from Veterinary Diagnostic Laboratories suggest an increasing incidence of cases occurring in the major regions of swine production in the U.S. Although previous studies reproduced polyarthritis by inoculating pigs with either Mycoplasma hyosynoviae or M. hyorhinis, there is a lack of information regarding the epidemiology of these microorganisms within swine populations. In addition, although new diagnostic tools have become available, information regarding the application and performance of these assays is lacking.
Therefore, a major objective of the work described in this thesis was to review and to provide new information regarding the use of diagnostic methods in the detection of M. hyosynoviae and M. hyorhinis. In order to evaluate the performance of a new real-time polymerase chain reaction (RT-PCR), a comparison of various DNA extraction methods using different clinical specimens was conducted in the laboratory. Thereafter, a field study was carried using RT-PCR- and antibody-based assays to monitor the infection dynamics of these microorganisms in a wean-to-finish population.
Joao Carlos Gomes Neto
Gomes Neto, Joao Carlos, "Diagnostic and field investigations in Mycoplasma hyosynoviae and Mycoplasma hyorhinis" (2012). Graduate Theses and Dissertations. 12969.