A commercial rubber waste product, fine rubber particle media (FRPM), was found to adsorb hydrogen sulfide gas (H2S) from biogas. Compared with traditional H2S adsorbents, economic advantages make FRPM a potentially attractive alternative. A number of experiments were conducted to investigate fundamental information, surface properties, and breakthrough characteristics of these innovative adsorbents. The physical properties, composition and surface chemistry were investigated and compared with commonly available commercial H2S adsorbents, such as activated carbon and metal oxide, in order to compare performance and to assess the possible adsorption mechanism.