Degree Type

Dissertation

Date of Award

2014

Degree Name

Doctor of Philosophy

Department

Horticulture

Major

Genetics

First Advisor

Shui-zhang Fei

Abstract

In this dissertation studies, BdCBF genes that are involved in cold acclimation in Brachypodium distachyon are identified and differentially expressed cold-responsive genes in cbf3 mutants are analyze by RNA-seq analysis. In addition, a high throughput RNAi library by the Phi29-amplified RNAi construct (PARC) method from a creeping bentgrass cDNA library with mRNA from salt-treated tissue is constructed. Generation of AsBri1 loss-of-function mutants using the PARC method in creeping bentgrass has validated the PARC system. And disruption of BR reception results in reduced growth along with increased drought tolerance in creeping bentgrass. Furthermore, the brassinosteroid signaling network including implications of brassinosteroid on yield and stress tolerance is reviewed. These genomic studies provide insights into the pathways involved in cold acclimation, BR signaling and plant stress responses.

Copyright Owner

Jingjie Hao

Language

en

File Format

application/pdf

File Size

155 pages

Archive.zip (2401 kB)
Raw data and gene list (spreadsheets)

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