Date of Award
Master of Science
Veterinary Diagnostic and Production Animal Medicine
Locke A. Karriker
To investigate a novel route for providing analgesia to processed piglets via transmammary drug delivery, meloxicam was administered orally to sows after farrowing. The objectives of this study were to prove the transmammary delivery of meloxicam from sows to piglets, describe initial pharmacokinetic parameters and to characterize the analgesic effects in piglets after processing through assessment of pain biomarkers and infrared thermography (IRT). Ten sows received either meloxicam (30 mg/kg) (n=5) or whey protein (placebo) (n=5) in their daily feedings, starting four days after farrowing and continuing for three consecutive days. During this period, blood and milk samples from sows and piglets were collected at 12-hour intervals. On Day 5 after farrowing, three boars and three gilts from each litter were castrated or sham castrated, tail docked and administered an iron injection. Piglet blood samples were collected immediately before processing and at predetermined times over an 84-hour period. IRT images were captured at each piglet blood collection point. Plasma was tested to confirm meloxicam concentrations using a validated high-performance liquid chromatography-mass spectrometry method. Meloxicam was detected in all piglets nursing on medicated sows at each time point, and the mean (±standard error of the mean) meloxicam concentration at castration was 568.9 ±105.8 ng/mL. Sow plasma and milk, along with piglet plasma reached steady concentrations at 36 hours after the administration of meloxicam. Furthermore, ex-vivo prostaglandin E2 (PGE2) synthesis inhibition was greater in piglets from treated sows compared to controls (p=0.0059). However, button gastric ulcers and subacute gastritis were noted in 2/5 meloxicam-treated sows and their litters. Initial sow oral meloxicam pharmacokinetic parameters (±SD) were calculated including absorption rate constant (ka) of 0.13 (0.05) hr-1, volume of distribution (V) 149.65 (8.94) mL/kg, and clearance (Cl) of 18.86 (0.45) mL/hr/kg. There was a time-by-treatment interaction for plasma cortisol (p=0.0009), with meloxicam-treated piglets demonstrating lower cortisol concentrations than control piglets for 10 hours after castration. No differences in mean plasma Substance P concentrations between treatment groups were observed (p=0.67). Lower cranial skin temperatures on IRT were observed in placebo compared to meloxicam-treated piglets (p=0.015).
Although there is great potential benefit to this method in the realms of analgesia, safety, and efficiency, dose refinement and a full understanding of pharmacokinetics of meloxicam by this route is needed before its application into swine.
Jessica Lea Bates
Bates, Jessica Lea, "Transmammary-delivered meloxicam in piglets undergoing castration and tail docking: impact on pharmacokinetics and pain biomarkers" (2015). Graduate Theses and Dissertations. 14754.