Degree Type

Thesis

Date of Award

2015

Degree Name

Master of Science

Department

Agronomy

Major

Genetics

First Advisor

Carolyn Lawrence-Dill

Second Advisor

Steven Cannon

Third Advisor

Jeffrey Wolt

Abstract

Researchers estimate that up to fifteen million Americans have food allergies. Of those affected, peanut allergies account for the highest number of deaths per year of any other food borne allergen. Although several peanut proteins have been identified for their role in triggering human immune response, there are four main proteins responsible for the hypersensitive reaction: Ara h 1, Ara h 2, Ara h 3, and Ara h 6. Anaphylaxis (an acute allergic reaction) is triggered by peanut in sensitive individuals, by hypersensitive immune response to the Ara h 2 protein, which has been found to be the most severely allergenic of the 4 genes. Here we use the CRISPR Genome Analysis Tool (CGAT) along with other bioinformatics techniques to propose a methodology for the targeted deletion of the Ara h 1 peanut allergen

DOI

https://doi.org/10.31274/etd-180810-4380

Copyright Owner

Vincent Antonio Brazelton Jr.

Language

en

File Format

application/pdf

File Size

49 pages

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