Date of Award
Master of Science
Researchers estimate that up to fifteen million Americans have food allergies. Of those affected, peanut allergies account for the highest number of deaths per year of any other food borne allergen. Although several peanut proteins have been identified for their role in triggering human immune response, there are four main proteins responsible for the hypersensitive reaction: Ara h 1, Ara h 2, Ara h 3, and Ara h 6. Anaphylaxis (an acute allergic reaction) is triggered by peanut in sensitive individuals, by hypersensitive immune response to the Ara h 2 protein, which has been found to be the most severely allergenic of the 4 genes. Here we use the CRISPR Genome Analysis Tool (CGAT) along with other bioinformatics techniques to propose a methodology for the targeted deletion of the Ara h 1 peanut allergen
Vincent Antonio Brazelton Jr.
Brazelton, Vincent Antonio Jr., "Enabling broad use of genome modification technologies to solve real world problems: a specific application in peanut" (2015). Graduate Theses and Dissertations. 14795.