Degree Type

Thesis

Date of Award

2016

Degree Name

Master of Science

Department

Materials Science and Engineering

Major

Materials Science and Engineering

First Advisor

Kaitlin Bratlie

Abstract

The effects of surface modifications on liposomes using a library of arginine derivatives for improved drug delivery were examined. Encapsulating either fluorescein or doxorubicin, both unmodified liposome and modified liposomes were tested for their drug delivery properties and propensity for internalization with macrophages. The modified liposomes were characterized by dynamic light scattering (DLS) and zeta potential. The resulting liposomes were able to encapsulate doxorubicin with a loading efficiency greater than 90% and cumulative releases of less than 15% after 144 h. The internalization of these particles was examined by loading the liposomes with fluorescein or doxorubicin to test internalization through fluorescence level and half maximal inhibitory concentration (IC50), respectively. Macrophages were activated with LPS or IL-4 to induce M1- or M2-like phenotypes. Naïve macrophages were also studied. Most modified liposomes enhanced the cytotoxicity of doxorubicin compared to unmodified liposomes. Macrophage phenotype was also observed to influence the cytotoxicity of the modified liposomes, with some modified liposomes enhancing the cytotoxicity in LPS stimulated macrophages and some enhancing IL-4 stimulated cells.

DOI

https://doi.org/10.31274/etd-180810-4642

Copyright Owner

Lilusi Ma

Language

en

File Format

application/pdf

File Size

47 pages

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