Degree Type

Thesis

Date of Award

2016

Degree Name

Master of Science

Department

Veterinary Microbiology and Preventive Medicine

Major

Veterinary Microbiology

First Advisor

Jianqiang Zhang

Abstract

Porcine epidemic diarrhea virus (PEDV) has been an important pathogen in Europe and Asia for several decades. It was identified in the United States (U.S.) swine population for the first time in April 2013, and spread rapidly across country and into Canada and Mexico. However, no information has been published regarding the minimum infectious dose (MID) of PEDV in different pig models. The main objective of this study was to determine the oral minimum infectious dose of PEDV in naïve conventional neonatal piglets and weaned pigs. A U.S. virulent PEDV prototype isolate (USA/IA19338/2013) with known infectious titer was serially ten-fold diluted in virus-negative cell culture medium, and inoculated into 5-day-old and 3-week-old pigs. Our data showed that PEDV is infectious in an age-dependent manner with a significantly lower MID for neonatal pigs compared to weaned pigs. Furthermore, it showed that, once an infection was established in pigs, the initial dose of PEDV administered did not affect the extent of fecal viral shedding, severity of histopathologic lesions, or magnitude of antibody titer subsequently developed. This information should be taken into consideration when interpreting clinical relevance of PEDV PCR results, designing a PEDV bioassay model, as well as emphasizes the importance of strict biosecurity and thorough cleaning/disinfection on sow farms.

DOI

https://doi.org/10.31274/etd-180810-4790

Copyright Owner

Joseph Tighe Thomas

Language

en

File Format

application/pdf

File Size

89 pages

Included in

Microbiology Commons

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