Degree Type

Dissertation

Date of Award

2016

Degree Name

Doctor of Philosophy

Department

Veterinary Microbiology and Preventive Medicine

Major

Microbiology

First Advisor

F. C. Minion

Abstract

ABSTRACT

The studies discussed herein were conducted to develop a vaccine for use in alleviating Mycoplasma hyorhinis associated disease in swine. This goal necessitated the development of a consistent and robust challenge model. We exploited M. hyorhinis’ affinity to infect tissue culture and have generated a cell-associated challenge material. This material was then administered in a consecutive-day, variable route model resulting in a high degree of lameness, arthritis and polyserositis in caesarian derived, colostrum deprived pigs. We further demonstrated an age limitation of the pigs for susceptibility to M. hyorhinis infection in this model. An inactivated whole-cell vaccine was developed and efficacy was evaluated in the challenge model. Vaccination provided significant reductions of pericarditis, arthritis and lameness as well as a significant increase in weight gain compared to a placebo control group. Lastly, a comparative proteomic analysis was performed between a broth-derived culture and two cell-associated cultures of a single isolate of M. hyorhinis. Differential expression of several membrane-associated proteins was observed in the cell-associated cultures. The identified proteins provide insight into the infection process and are targets for future vaccine trials.

DOI

https://doi.org/10.31274/etd-180810-5394

Copyright Owner

Brian Thomas Martinson

Language

en

File Format

application/pdf

File Size

177 pages

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