Degree Type

Thesis

Date of Award

2016

Degree Name

Master of Science

Department

Biomedical Sciences

Major

Immunobiology

First Advisor

Michael W. Cho

Abstract

Human Immunodeficiency Virus type 1 (HIV-1) is a highly variable virus that continues to cause a worldwide pandemic. The search for a vaccine has focused on the elicitation of broadly neutralizing antibodies (bnAbs), which can prevent the attachment and/or entry of the majority of HIV strains. In this thesis, we attempt to elicit more common neutralizing antibodies (nAbs), which are type-specific in their ability to block HIV infection. However, we propose to elicit a wide range of these type-specific nAbs to mimic the breadth of protection found in true bnAbs to protect the host from multiple HIV strains. We immunized both mice and rabbits with a twelve virus panel, termed global panel, that represents seven different HIV-1 clades. The immunizations were performed using particle mediated epidermal delivery (PMED) loaded with plasmids that could express the desired env proteins in vivo. The mice did not elicit any neutralizing activity against tier 1 or tier 2 viruses. The rabbits had neutralization against one of the tier 1 viruses tested, but not against any of the tier 2 global panel viruses.

Copyright Owner

Kari Loni Rohl

Language

en

File Format

application/pdf

File Size

57 pages

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