Date of Award
Master of Science
Michael W. Cho
Human Immunodeficiency Virus type 1 (HIV-1) is a highly variable virus that continues to cause a worldwide pandemic. The search for a vaccine has focused on the elicitation of broadly neutralizing antibodies (bnAbs), which can prevent the attachment and/or entry of the majority of HIV strains. In this thesis, we attempt to elicit more common neutralizing antibodies (nAbs), which are type-specific in their ability to block HIV infection. However, we propose to elicit a wide range of these type-specific nAbs to mimic the breadth of protection found in true bnAbs to protect the host from multiple HIV strains. We immunized both mice and rabbits with a twelve virus panel, termed global panel, that represents seven different HIV-1 clades. The immunizations were performed using particle mediated epidermal delivery (PMED) loaded with plasmids that could express the desired env proteins in vivo. The mice did not elicit any neutralizing activity against tier 1 or tier 2 viruses. The rabbits had neutralization against one of the tier 1 viruses tested, but not against any of the tier 2 global panel viruses.
Kari Loni Rohl
Rohl, Kari Loni, "Antibody response of mice and rabbits targeting HIV-1 using global panel DNA and SOSIP gp140 protein" (2016). Graduate Theses and Dissertations. 15800.