Date of Award
Master of Science
Agricultural and Biosystems Engineering
Thomas B. Moorman
Michelle L. Soupir
The application of swine manure to agricultural fields increases the abundance of antibiotic resistance genes (ARG) and antibiotic resistant bacteria (ARB) in soils. While manure-associated antibiotic resistant bacteria persist following application, there is potential for their transport through tile drainage. The environmental survival of bacteria in soil has been related to moisture and temperature, therefore differences in these environmental conditions between spring and fall soils may cause manure associated antibiotic resistance to behave differently following application. This encouraged exploring differences in the persistence of antibiotic resistant bacteria and antibiotic resistance genes in soil when manure is applied at different times of the year. In this paper, two analyses determined the persistence of five measures of antibiotic resistance in five agricultural systems. Half-lives and least-squared means of ARB and ARG in manured soils are compared to determine differences in ARB and ARG persistence between agricultural practices. Corn-soybean systems receiving manure in the early fall had longer half-lives than similar systems receiving manure in the late fall. This research would serve as a recommendation for agricultural practices which minimize the persistence of swine-manure associated antibiotic resistance.
Elliot George Rossow
Rossow, Elliot George, "The effect of manure application timing on the persistence of phenotypic and genotypic antibiotic resistance in tile-drained hapludolls" (2018). Graduate Theses and Dissertations. 16872.