Degree Type


Date of Award


Degree Name

Master of Science


Agricultural and Biosystems Engineering


Environmental Science

First Advisor

Thomas B. Moorman

Second Advisor

Michelle L. Soupir


The application of swine manure to agricultural fields increases the abundance of antibiotic resistance genes (ARG) and antibiotic resistant bacteria (ARB) in soils. While manure-associated antibiotic resistant bacteria persist following application, there is potential for their transport through tile drainage. The environmental survival of bacteria in soil has been related to moisture and temperature, therefore differences in these environmental conditions between spring and fall soils may cause manure associated antibiotic resistance to behave differently following application. This encouraged exploring differences in the persistence of antibiotic resistant bacteria and antibiotic resistance genes in soil when manure is applied at different times of the year. In this paper, two analyses determined the persistence of five measures of antibiotic resistance in five agricultural systems. Half-lives and least-squared means of ARB and ARG in manured soils are compared to determine differences in ARB and ARG persistence between agricultural practices. Corn-soybean systems receiving manure in the early fall had longer half-lives than similar systems receiving manure in the late fall. This research would serve as a recommendation for agricultural practices which minimize the persistence of swine-manure associated antibiotic resistance.

Copyright Owner

Elliot George Rossow



File Format


File Size

94 pages