Degree Type

Thesis

Date of Award

2020

Degree Name

Master of Science

Department

Electrical and Computer Engineering

Major

Electrical Engineering (Microelectronics and photonics)

First Advisor

Meng Lu

Abstract

This work proposes a paper-based sensor system for point-of-care nucleic acid amplification tests. The system integrates a paper sensor chip and a compact fluorescence detector, which is built towards the Internet of Things (IoT) platform via an Arduino controller. The paper-based sensor provides the functions of reagent storage, sample transportation, and DNA amplification. The compact and low-cost fluorescence detector is capable of quantifying the amplicons of the loop-mediated isothermal amplification (LAMP) reaction in real-time. The results show that the paper-based sensor can detect multiple genes of the genomic DNA extracted from Escherichia coli and Campylobacter jejuni, with the concentration as low as 2 × 103 copies/μL. As a diagnosis tool, the sensor has the potential to measure multiple target genes simultaneously.

DOI

https://doi.org/10.31274/etd-20200902-90

Copyright Owner

Mingdian Liu

Language

en

File Format

application/pdf

File Size

62 pages

Available for download on Sunday, February 28, 2021

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