Degree Type
Thesis
Date of Award
2020
Degree Name
Master of Science
Department
Electrical and Computer Engineering
Major
Electrical Engineering (Microelectronics and photonics)
First Advisor
Meng Lu
Abstract
This work proposes a paper-based sensor system for point-of-care nucleic acid amplification tests. The system integrates a paper sensor chip and a compact fluorescence detector, which is built towards the Internet of Things (IoT) platform via an Arduino controller. The paper-based sensor provides the functions of reagent storage, sample transportation, and DNA amplification. The compact and low-cost fluorescence detector is capable of quantifying the amplicons of the loop-mediated isothermal amplification (LAMP) reaction in real-time. The results show that the paper-based sensor can detect multiple genes of the genomic DNA extracted from Escherichia coli and Campylobacter jejuni, with the concentration as low as 2 × 103 copies/μL. As a diagnosis tool, the sensor has the potential to measure multiple target genes simultaneously.
DOI
https://doi.org/10.31274/etd-20200902-90
Copyright Owner
Mingdian Liu
Copyright Date
2020-08
Language
en
File Format
application/pdf
File Size
62 pages
Recommended Citation
Liu, Mingdian, "An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests" (2020). Graduate Theses and Dissertations. 18171.
https://lib.dr.iastate.edu/etd/18171
