Degree Type

Thesis

Date of Award

2020

Degree Name

Doctor of Philosophy

Department

Physics and Astronomy

Major

Physics

First Advisor

Xuefeng Wang

Abstract

Cell migration is a fundamental cellular function for many physiological processes and normal animal development. In order to migrate, cells produce forces to interact with extra cellular environments, mostly via integrin, a family of transmembrane proteins. The distribution and dynamics of integrins at different parts of cells should be well tuned for directional and coordinated migration.In order to detect cellular force distribution, researchers have developed different tools for cellular force detection. One of them is DNA-based tension sensor. This type of sensors have the advantage of high spatial resolution at optical limits, high temporal sensitivity at submicron and simplicity for usage. However, DNA-based tension sensors suffer from the susceptibility of DNase and their conventional spatial resolution is limited to optical limits. In this dissertation, I will introduce 3 projects during my Ph.D. period. Firstly, we used integrative tension sensor (ITS) to detect the cellular force distribution of fast migratory fish keratocytes and found the cellular force is utilized for adhesion rear deadhesion. Then, we designed and developed DNase-resistant tension sensor that is both resistant to DNase and capable of reporting cellular force. Lastly, we created cellular force nanoscopy that images cellular force with 50nm resolution.

DOI

https://doi.org/10.31274/etd-20210114-169

Copyright Owner

Yuanchang Zhao

Language

en

File Format

application/pdf

File Size

121 pages

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