Degree Type

Thesis

Date of Award

2021

Degree Name

Master of Science

Department

Biomedical Sciences

Major

Biomedical Sciences

First Advisor

Jonathan P Mochel

Abstract

Bladder Cancer is the 9th most common malignancy in the world. Transitional cell carcinoma (TCC) is the most common of bladder cancers, occurring in 90% of cases. There has been no great model established to study TCC in vitro. In this study, we explore urine-derived, 3-dimensional, canine TCC organoids as a possible model to study TCC in vitro. After establishing the cell line, we subjected the 3-D cells to RNA in situ hybridization (RNAish) and cell viability assays. Overall, 3-D cell culture from urine samples of TCC diagnosed canines expressed RNA biomarkers in a similar manner to parent tumors via RNAish and showed more sensitivity to Cisplatin treatment when compared to 2-D human TCC cells. With further experimentation, canine TCC organoids could be an ideal model to study TCC in vitro.

DOI

https://doi.org/10.31274/etd-20210609-187

Copyright Owner

Savantha Thenuwara

Language

en

File Format

application/pdf

File Size

24 pages

Share

COinS