This project investigated field production of a potential biofuel crop–sugar beet. Objectives included becoming familiar with cultural methods for growing sugar beet and determining expected yield levels for southeast Iowa. To accomplish this, half-acre trial plots were planted at the Muscatine Island Research Farm (MIRF), Fruitland and at the Southeast Research Farm (SERF), Crawfordsville, in 2008 and 2009. The two sites provided different soil types and growing environments to investigate the crop. Results from both years and locations demonstrate that sugar beets can be grown in southeast Iowa, and with good management and weather conditions, yields should equal or exceed the national average of 26.0 ton/acre.