Document Type
Article
Publication Date
6-10-2000
Journal or Book Title
Journal of Agricultural and Food Chemistry
Volume
48
Issue
7
First Page
2702
Last Page
2708
DOI
10.1021/jf990785w
Abstract
A simplification of the pilot-plant scale modified Nagano method yielding two protein fractions, glycinin and â-conglycinin, by pH adjustment and ultrafiltration membrane separation was developed and compared with our pilot-plant-scale modified Nagano procedure and with a soy protein isolate pilot-plant procedure as our reference process. Two protein fractions, glycinin and â-conglycinin, were produced from our simplified process and compared to the three protein fractions, glycinin, â-conglycinin, and an intermediate protein mixture, produced with the modified Nagano method. The pilot-plant yields of glycinin, â-conglycinin, and intermediate mixture fractions from the modified Nagano method were 9.4, 10.3, and 4.8% [dry basis (db)], respectively. The yield of glycinin fraction of the simplified method was 9.7% (db), and it had a protein content and purity similar to those obtained with the modified Nagano method. The yield of the â-conglycinin fraction was 19.6% (db), which was twice that of the modified Nagano process. The protein content of â-conglycinin was 91.6% (db), and the purity was 62.6% of the protein content, which was 9% lower in purity than the modified Nagano method. Process optimization of the simplified method suggested the best operating conditions for the membrane filtration system were 20-25 psi inlet pressure and 200-250 L/min ultrafiltration recirculation speeds.
Rights
One-time permission is granted only for the use specified in your request. No additional uses are granted (such as derivative works or other editions).
Copyright Owner
American Chemical Society
Copyright Date
2000
Language
en
File Format
application/pdf
Recommended Citation
Wu, Shaowen; Murphy, Patricia A.; Johnson, Lawrence A.; Reuber, Mark A.; and Fratzke, Alfred R., "Simplified Process for Soybean Glycinin and â-Conglycinin Fractionation" (2000). Food Science and Human Nutrition Publications. 103.
https://lib.dr.iastate.edu/fshn_ag_pubs/103
Included in
Agricultural Science Commons, Food Science Commons, Human and Clinical Nutrition Commons, Plant Pathology Commons
Comments
Reprinted with permission from Journal of Agricultural and Food Chemistry . 48(7): 2702-2708. doi: 10.1021/jf990785w. Copyright 2000 American Chemical Society.