Journal or Book Title
Journal of Agricultural and Food Chemistry
A simplification of the pilot-plant scale modified Nagano method yielding two protein fractions, glycinin and â-conglycinin, by pH adjustment and ultrafiltration membrane separation was developed and compared with our pilot-plant-scale modified Nagano procedure and with a soy protein isolate pilot-plant procedure as our reference process. Two protein fractions, glycinin and â-conglycinin, were produced from our simplified process and compared to the three protein fractions, glycinin, â-conglycinin, and an intermediate protein mixture, produced with the modified Nagano method. The pilot-plant yields of glycinin, â-conglycinin, and intermediate mixture fractions from the modified Nagano method were 9.4, 10.3, and 4.8% [dry basis (db)], respectively. The yield of glycinin fraction of the simplified method was 9.7% (db), and it had a protein content and purity similar to those obtained with the modified Nagano method. The yield of the â-conglycinin fraction was 19.6% (db), which was twice that of the modified Nagano process. The protein content of â-conglycinin was 91.6% (db), and the purity was 62.6% of the protein content, which was 9% lower in purity than the modified Nagano method. Process optimization of the simplified method suggested the best operating conditions for the membrane filtration system were 20-25 psi inlet pressure and 200-250 L/min ultrafiltration recirculation speeds.
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American Chemical Society
Wu, Shaowen; Murphy, Patricia A.; Johnson, Lawrence A.; Reuber, Mark A.; and Fratzke, Alfred R., "Simplified Process for Soybean Glycinin and â-Conglycinin Fractionation" (2000). Food Science and Human Nutrition Publications. 103.