Document Type
Article
Publication Date
1-11-2012
Journal or Book Title
Journal of Agricultural and Food Chemistry
Volume
60
Issue
1
First Page
451
Last Page
457
DOI
10.1021/jf203629w
Abstract
An effective process for the enzymatic synthesis of oleoylethanolamide is described in this study. The process included purification of a commercial oleic acid product and then optimization of the reaction between the purified oleic acid and ethanolamine in the presence of hexane and a lipase. Under the optimal amidation reaction conditions identified, oleoylethanolamide was obtained with 96.6% purity. The synthesis was also conducted on a large scale (50 mmol of each of the reactants), and oleoylethanolamide purity and yield after crystallization purification were 96.1 and 73.5%, respectively. Compared to the previous studies, the current method of preparing high-purity oleoylethanolamide is more effective and economically feasible. The scalability and ease for such synthesis make it possible to study the biological and nutritional functions of the cannabinoid-like oleoylethanolamide in animal or human subjects.
Copyright Owner
American Chemical Society
Copyright Date
2011
Language
en
File Format
application/pdf
Recommended Citation
Wang, Xiaosan; Wang, Xingguo; and Wang, Tong, "Synthesis of Oleoylethanolamide Using Lipase" (2012). Food Science and Human Nutrition Publications. 14.
https://lib.dr.iastate.edu/fshn_ag_pubs/14
Comments
Posted with permission from Journal of Agricultural and Food Chemistry,60, no. 1 (2012): 451–457, doi: 10.1021/jf203629w. Copyright 2011 American Chemical Society.