Campus Units

Food Science and Human Nutrition

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

12-2015

Journal or Book Title

Industrial Crops and Products

Volume

77

Issue

23

First Page

803

Last Page

808

DOI

10.1016/j.indcrop.2015.09.060

Abstract

Application of hydrolytic and other enzymes for improving fermentation performance and oil recovery in corn dry-grind process was optimized. Non-starch polysaccharide enzymes (BluZy-P XL; predominantly xylanase activity) were added at stages prior to fermentation at optimum conditions of 50 ◦C and pH 5.2 and compared with conventional fermentation (30 ◦C, pH 4.0). Enzyme applications resulted in faster ethanol production rates with a slight increase in yield compared to control. The thin stillage yield increased by 0.7–5% w/w wet basis with corresponding increase in solids content with enzyme treatment after liquefaction. The oil partitioned in thin stillage was at 67.7% dry basis after treatment with hydrolytic enzymes during fermentation. Further addition of protease and phytase during simultaneous saccharification and fermentation increased thin stillage oil partitioning to 77.8%. It also influenced other fermentation parameters, e.g., ethanol production rate increased to 1.16 g/g dry corn per hour, and thin stillage wet solids increased by 2% w/w. This study indicated that treatments with non-starch hydrolytic enzymes have potential to improve the performance of corn dry-grind process including oil partitioning into thin stillage. The novelty of this research is the addition of protease and phytase enzymes during simultaneous saccharification and fermentation of corn dry-grind process, which further improved ethanol yields and oil partitioning into thin stillage.

Comments

This accepted article is published as Luangthongkam, P., Fang, L., Noomhorm, A., Lamsal, B.* 2015. Addition of hydrolytic enzymes and phytase for improving fermentation performance and oil recovery in dry-grind ethanol process, Industrial Crops and Products, 77: 803–808. DOI: 10.1016/j.indcrop.2015.09.060. Posted with permission.

Copyright Owner

Elsevier Ltd

Language

en

File Format

application/pdf

Published Version

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