Campus Units
Food Science and Human Nutrition
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
12-2015
Journal or Book Title
Industrial Crops and Products
Volume
77
Issue
23
First Page
803
Last Page
808
DOI
10.1016/j.indcrop.2015.09.060
Abstract
Application of hydrolytic and other enzymes for improving fermentation performance and oil recovery in corn dry-grind process was optimized. Non-starch polysaccharide enzymes (BluZy-P XL; predominantly xylanase activity) were added at stages prior to fermentation at optimum conditions of 50 ◦C and pH 5.2 and compared with conventional fermentation (30 ◦C, pH 4.0). Enzyme applications resulted in faster ethanol production rates with a slight increase in yield compared to control. The thin stillage yield increased by 0.7–5% w/w wet basis with corresponding increase in solids content with enzyme treatment after liquefaction. The oil partitioned in thin stillage was at 67.7% dry basis after treatment with hydrolytic enzymes during fermentation. Further addition of protease and phytase during simultaneous saccharification and fermentation increased thin stillage oil partitioning to 77.8%. It also influenced other fermentation parameters, e.g., ethanol production rate increased to 1.16 g/g dry corn per hour, and thin stillage wet solids increased by 2% w/w. This study indicated that treatments with non-starch hydrolytic enzymes have potential to improve the performance of corn dry-grind process including oil partitioning into thin stillage. The novelty of this research is the addition of protease and phytase enzymes during simultaneous saccharification and fermentation of corn dry-grind process, which further improved ethanol yields and oil partitioning into thin stillage.
Copyright Owner
Elsevier Ltd
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Luangthongkam, P.; Fang, L.; Noomhorm, A.; and Lamsal, Buddhi P., "Addition of cellulolytic enzymes and phytase for improving ethanol fermentation performance and oil recovery in corn dry grind process" (2015). Food Science and Human Nutrition Publications. 200.
https://lib.dr.iastate.edu/fshn_ag_pubs/200
Included in
Food Chemistry Commons, Food Processing Commons, Human and Clinical Nutrition Commons, Molecular, Genetic, and Biochemical Nutrition Commons
Comments
This accepted article is published as Luangthongkam, P., Fang, L., Noomhorm, A., Lamsal, B.* 2015. Addition of hydrolytic enzymes and phytase for improving fermentation performance and oil recovery in dry-grind ethanol process, Industrial Crops and Products, 77: 803–808. DOI: 10.1016/j.indcrop.2015.09.060. Posted with permission.