Campus Units

Food Science and Human Nutrition, Center for Crops Utilization Research

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2016

Journal or Book Title

Bioprocess and Biosystems Engineering

Volume

39

Issue

1

First Page

105

Last Page

113

DOI

10.1007/s00449-015-1494-4

Abstract

Pretreatment of six fibrous biomasses (switchgrass, alfalfa, soy hulls, soy fiber, DDGS and Baggase) and subsequent hydrolysis using cellulolytic enzymes at a 2.5% (v/v) and 5% (v/v) loading 2.5 (v/v) and 5% (v/v) loading was compared for higher amounts of sugars released. Soaking of biomasses of switchgrass, alfalfa, soy hulls and bagasse in 15% w/w ammonia was optimal at 60 °C for 12 h, followed by enzymatic hydrolysis, yielding 72, 70, 80 and 75% carbohydrate conversions, respectively. However, soaking in ammonia was not needed for soy fiber and DDGS as these contained very little lignin. Ultrasonication for 3 min @ 100% amplitude (170 µM) was found to be optimal for soy fiber and DDGS from which 77 and 83% carbohydrate conversion, respectively, was obtained following enzyme treatment at 5% (w/v) enzyme. The sugars released by enzymatic hydrolysis of pretreated biomass were utilized as an energy source by Bacillus subtilis in fermentation media at 2% (w/v) of concentration. In shake flask trials, cell growth was 15-20% higher on hydrolysates of ammonia-treated switchgrass and alfalfa vs. glucose-based control media due to the presence of a wider range of monomeric sugars (glucose, xylose, arabinose, mannose and galactose). In contrast, growth was less on soy hull hydrolysates prepared with ammonia pretreatment.

Comments

This accepted article is published as Sharma, R., Lamsal, B. P.*, Colonna, W.J. 2016. Pretreatment of fibrous biomass and growth of biosurfactant-producing Bacillus subtilis on biomass-derived fermentable sugars, Bioprocess and Biosystems Engineering, 39(1): 105-113. DOI: 10.1007/s00449-015-1494-4. Posted with permission.

Copyright Owner

Springer-Verlag Berlin Heidelberg

Language

en

File Format

application/pdf

Published Version

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