Document Type
Article
Publication Date
2010
Journal or Book Title
Journal of Agricultural and Food Chemistry
Volume
58
Issue
15
First Page
8573
Last Page
8584
DOI
10.1021/jf1014268
Abstract
Bauer alkylamide 11 and Bauer ketone 23 were previously found to be partially responsible forEchinacea angustifolia anti-inflammatory properties. This study further tested their importance using the inhibition of prostaglandin E2 (PGE2) and nitric oxide (NO) production by RAW264.7 mouse macrophages in the absence and presence of lipopolysaccharide (LPS) and E. angustifolia extracts, phytochemical enriched fractions, or pure synthesized standards. Molecular targets were probed using microarray, qRT-PCR, Western blot, and enzyme assays. Fractions with these phytochemicals were more potent inhibitors of LPS-induced PGE2 production than E. angustifolia extracts. Microarray did not detect changes in transcripts with phytochemical treatments; however, qRT-PCR showed a decrease in TNF-α and an increase of iNOS transcripts. LPS-induced COX-2 protein was increased by an E. angustifolia fraction containing Bauer ketone 23 and by pure phytochemical. COX-2 activity was decreased with all treatments. The phytochemical inhibition of PGE2 production byEchinacea may be due to the direct targeting of COX-2 enzyme.
Copyright Owner
American Chemical Society
Copyright Date
2010
Language
en
File Format
application/pdf
Recommended Citation
LaLone, Carlie A.; Huang, Nan; Rizshsky, Ludmila; Yum, Man-Yu; Singh, Navrozedeep; Hauck, Cathy; Nikolau, Basil J.; Wurtele, Eve S.; Kohut, Marian L.; Murphy, Patricia A.; and Birt, Diane F., "Enrichment of Echinacea angustifolia with Bauer Alkylamide 11 and Bauer Ketone 23 Increased Anti-inflammatory Potential through Interference with COX-2 Enzyme Activity" (2010). Food Science and Human Nutrition Publications. 23.
https://lib.dr.iastate.edu/fshn_ag_pubs/23
Included in
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Comments
Posted with permission from Journal of Agricultural and Food Chemistry 58, no. 15 (2010): 8573–8584, doi:10.1021/jf1014268. Copyright 2010 American Chemical Society.