Campus Units
Food Science and Human Nutrition, Biochemistry, Biophysics and Molecular Biology, Horticulture, Agronomy
Document Type
Article
Publication Date
2-2012
Journal or Book Title
Phytochemistry
Volume
74
First Page
146
Last Page
158
DOI
10.1016/j.phytochem.2011.10.013
Abstract
Among the nine Echinacea species, E. purpurea, E. angustifolia and E. pallida, have been widely used to treat the common cold, flu and other infections. In this study, ethanol extracts of these three Echinaceaspecies and E. paradoxa, including its typical variety, E. paradoxa var. paradoxa, were screened in lipopolysaccharide (LPS)-stimulated macrophage cells to assess potential anti-inflammatory activity. E. paradoxa var. paradoxa, rich in polyenes/polyacetylenes, was an especially efficient inhibitor of LPS-induced production of nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) by 46%, 32%, 53% and 26%, respectively, when tested at 20 μg/ml in comparison to DMSO control. By bioactivity-guided fractionation, pentadeca-8Z-ene-11, 13-diyn-2-one (Bauer ketone 23) and pentadeca-8Z, 13Z-dien-11-yn-2-one (Bauer ketone 24) from E. paradoxa var. paradoxa were found primarily responsible for inhibitory effects on NO and PGE2 production. Moreover, Bauer ketone 24 was the major contributor to inhibition of inflammatory cytokine production in LPS-induced mouse macrophage cells. These results provide a rationale for exploring the medicinal effects of the Bauer ketone-rich taxon, E. paradoxa var.paradoxa, and confirm the anti-inflammatory properties of Bauer ketones 23 and 24.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Zhang, Xiaozhu; Rizshsky, Ludmila; Hauck, Catherine C.; Qu, Luping; Widrlechner, Mark P.; Nikolau, Basil J.; Murphy, Patricia A.; and Birt, Diane F., "Bauer ketones 23 and 24 from Echinacea paradoxa var. paradoxa inhibit lipopolysaccharide-induced nitric oxide, prostaglandin E2 and cytokines in RAW264.7 mouse macrophages" (2012). Food Science and Human Nutrition Publications. 37.
https://lib.dr.iastate.edu/fshn_ag_pubs/37
Included in
Biochemistry Commons, Developmental Biology Commons, Food Science Commons, Human and Clinical Nutrition Commons, Molecular Biology Commons
Comments
This article is from Phytochemistry 74 (February 2012): 146–158, doi:10.1016/j.phytochem.2011.10.013.