Document Type
Article
Publication Date
10-8-2005
Journal or Book Title
Journal of Agricultural and Food Chemistry
Volume
53
Issue
22
First Page
8519
Last Page
8525
DOI
10.1021/jf051546d
Abstract
Gut microbial disappearance and metabolism of the soy isoflavone glycitein, 7,4‘-dihydroxy-6-methoxyisoflavone, were investigated by incubating glycitein anaerobically with feces from 12 human subjects. The subjects' ages ranged from 24 to 53 years with a body mass index (BMI) of 20.9−25.8 kg/m2 (mean BMI = 24.0 ± 1.1 kg/m2). Glycitein disappearance followed an apparent first-order rate loss. Fecal glycitein disappearance rates for the subjects segregated into three different groups described as high (k = 0.67 ± 0.14/h), moderate (k = 0.34 ± 0.04/h), and low (k = 0.15 ± 0.07/h) glycitein degraders (p < 0.0001). There was no dose effect on the disappearance rates for each subject from 10 to 250 μM glycitein (averagek = 0.32 ± 0.03/h, p > 0.05). Four putative glycitein metabolites, characterized by liquid chromatography−mass spectrometry (electrospray ionization using positive ionization mode), were dihydroglycitein, dihydro-6,7,4‘-trihydroxyisoflavone, and 5‘-O-methyl-O-desmethylangolensin. Two subjects produced a metabolite tentatively identified as 6-O-methyl-equol, and one subject produced daidzein as an additional metabolite of glycitein. These results show that glycitein is metabolized by human gut microorganisms and may follow metabolic pathways similar to other soy isoflavones.
Copyright Owner
American Chemical Society
Copyright Date
2005
Language
en
File Format
application/pdf
Recommended Citation
Simons, Andrean L.; Renouf, Mathieu; Hendrich, Suzanne; and Murphy, Patricia A., "Metabolism of Glycitein (7,4-Dihydroxy-6-methoxy-isoflavone) by Human Gut Microflora" (2005). Food Science and Human Nutrition Publications. 81.
https://lib.dr.iastate.edu/fshn_ag_pubs/81
Comments
Reprinted with permission from Journal of Agricultural and Food Chemistry, 53(22):8519-8525. doi: 10.1021/jf051546d . Copyright 2005 American Chemical Society.