Campus Units
Genetics, Development and Cell Biology, Agronomy
Document Type
Article
Publication Version
Published Version
Publication Date
9-28-2011
Journal or Book Title
Nucleic Acids Research
Volume
39
Issue
22
First Page
e149
DOI
10.1093/nar/gkr718
Abstract
Previous studies have shown that pairs of closely-linked Ac/Ds transposable elements can induce various chromosomal rearrangements in plant genomes. To study chromosomal rearrangements in rice, we isolated a line ( OsRLG5-161) that contains two inversely-oriented Ds insertions in OsRLG5 ( Oryza sativa Receptor like kinase Gene 5). Among approximately 300 plants regenerated from OsRLG5-161 heterozygous seeds, 107 contained rearrangements including deletions, duplications and inversions of various sizes. Most rearrangements were induced by previously identified alternative transposition mechanism. Furthermore, we also detected a new class of rearrangements that contain juxtaposed inversions and deletions on the same chromosome. We propose that these novel alleles were generated by a previously unreported type of alternative transposition reactions involving the 5′ and 3′ termini of two inversely-oriented Ds elements located on the same chromatid. Finally, 11% of rearrangements contained inversions resulting from homologous recombination between the two inverted Ds elements in OsRLG5-161 . The high frequency inheritance and great variety of rearrangements obtained suggests that the rice regeneration system results in a burst of transposition activity and a relaxation of the controls which normally limit the transposition competence of individual Ds termini. Together, these results demonstrate a greatly enlarged potential of the Ac/Ds system for plant chromosome engineering.
Rights
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright Owner
The Authors
Copyright Date
2011
Language
en
File Format
application/pdf
Recommended Citation
Xuan, Yuan Hu; Piao, Hai Long; Je, Byoung Il; Park, Soon Ju; Huang, Jin; Zhang, Jian Bo; Peterson, Thomas; and Han, Chang-deok, "Transposon Ac/Ds -induced chromosomal rearrangements at the rice OsRLG5 locus" (2011). Genetics, Development and Cell Biology Publications. 134.
https://lib.dr.iastate.edu/gdcb_las_pubs/134
Included in
Agronomy and Crop Sciences Commons, Cell and Developmental Biology Commons, Genetics and Genomics Commons, Plant Breeding and Genetics Commons
Comments
This article is from Nucleic Acids Research 39 (2011): e149, doi: 10.1093/nar/gkr718. Posted with permission.