Campus Units
Genetics, Development and Cell Biology, Agronomy, Statistics, Plant Pathology and Microbiology, Virtual Reality Applications Center
Document Type
Article
Publication Version
Published Version
Publication Date
7-2016
Journal or Book Title
Genetics
Volume
203
Issue
3
First Page
1133
Last Page
1147
DOI
10.1534/genetics.116.188235
Abstract
Copy-number alterations are widespread in animal and plant genomes, but their immediate impact on gene expression is still unclear. In animals, copy-number alterations usually exhibit dosage effects, except for sex chromosomes which tend to be dosage compensated. In plants, genes within small duplications (<100 kb) often exhibit dosage-dependent expression, whereas large duplications (>50 Mb) are more often dosage compensated. However, little or nothing is known about expression in moderately-sized (1–50 Mb) segmental duplications, and about the response of small RNAs to dosage change. Here, we compared maize (Zea mays) plants with two, three, and four doses of a 14.6-Mb segment of chromosome 1 that contains ∼300 genes. Plants containing the duplicated segment exhibit dosage-dependent effects on ear length and flowering time. Transcriptome analyses using GeneChip and RNA-sequencing methods indicate that most expressed genes and unique small RNAs within the duplicated segments exhibit dosage-dependent transcript levels. We conclude that dosage effect is the predominant regulatory response for both genes and unique small RNA transcripts in the segmental dosage series we tested. To our knowledge this is the first analysis of small RNA expression in plant gene dosage variants. Because segmental duplications comprise a significant proportion of eukaryotic genomes, these findings provide important new insight into the regulation of genes and small RNAs in response to dosage changes.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Zuo, Tao; Zhang, Jianbo; Lithio, Andrew; Dash, Sudhansu; Weber, David F.; Wise, Roger; Nettleton, Dan; and Peterson, Thomas, "Genes and Small RNA Transcripts Exhibit Dosage-Dependent Expression Pattern in Maize Copy-Number Alterations" (2016). Genetics, Development and Cell Biology Publications. 137.
https://lib.dr.iastate.edu/gdcb_las_pubs/137
Included in
Agronomy and Crop Sciences Commons, Plant Breeding and Genetics Commons, Statistical Methodology Commons
Comments
This article is from Genetics 203 (2016): 1133, doi: 10.1534/genetics.116.188235.