Campus Units
Genetics, Development and Cell Biology, Biomedical Sciences, Neuroscience, Veterinary Clinical Sciences, Biochemistry, Biophysics and Molecular Biology
Document Type
Article
Publication Version
Published Version
Publication Date
2008
Journal or Book Title
Journal of Histochemistry & Cytochemistry
Volume
56
Issue
9
First Page
863
Last Page
872
Abstract
This study provides a detailed description of immunolocalization of two oxygen-binding proteins, neuroglobin (Ngb) and cytoglobin (Cygb), in the anterior segment of healthy human and canine eyes. Specific antibodies against Ngb and Cygb were used to examine their distribution patterns in anterior segment structures including the cornea, iris, trabecular meshwork, canal of Schlemm, ciliary body, and lens. Patterns of immunoreactivity (IR) were imaged with confocal scanning laser and conventional microscopy. Analysis of sectioned human and canine eyes showed Ngb and Cygb IR in the corneal epithelium and endothelium. In the iris, Ngb and Cygb IR was localized to the anterior border and the stroma, iridal sphincter, and dilator muscle. In the iridocorneal angle, Ngb and Cygb were detected in endothelial cells of the trabecular meshwork and canal of Schlemm in human. In the ciliary body, Ngb and Cygb IR was localized to the non-pigmented ciliary epithelium of the pars plana and pars plicata and in ciliary body musculature. Ngb and Cygb distribution was similar and colocalized within the same structures of healthy human and canine anterior eye segments. Based on their immunolocalization and previously reported biochemical features, we hypothesize that Ngb and Cygb may function as scavengers of reactive oxygen species. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Ostojić, Jelena; Grozdanić, Siniša; Syed, Nasreen A.; Hargrove, Mark S.; Trent, James T. III; Kuehn, Markus H.; Kardon, Randy H.; Kwon, Young H.; and Sakaguchi, Donald S., "Neuroglobin and Cytoglobin Distribution in the Anterior Eye Segment: A Comparative Immunohistochemical Study" (2008). Genetics, Development and Cell Biology Publications. 143.
https://lib.dr.iastate.edu/gdcb_las_pubs/143
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Cell and Developmental Biology Commons, Comparative and Laboratory Animal Medicine Commons
Comments
This article is from Journal of Histochemistry & Cytochemistry 56 (2008): 863.