Campus Units
Genetics, Development and Cell Biology, Biomedical Sciences
Document Type
Article
Publication Version
Published Version
Publication Date
9-2012
Journal or Book Title
Veterinary Ophthalmology
Volume
15
Issue
s2
First Page
94
Last Page
104
DOI
10.1111/j.1463-5224.2012.01034.x
Abstract
Objective To perform in vivo analysis of retinal functional and structural parameters in healthy mouse eyes.
Animal Studied Adult C57BL/6 male mice (n = 37).
Procedures Retinal function was evaluated using pattern electroretinography (pERG) and the chromatic pupil light reflex (cPLR). Structural properties of the retina and nerve fiber layer (NFL) were evaluated using spectral-domain optical coherence tomography (SD-OCT).
Results The average pERG amplitudes were found to be 11.2 ± 0.7 μV (P50-N95, mean ± SEM), with an implicit time for P50-N95 interval of 90.4 ± 5.4 ms. Total retinal thickness was 229.5 ± 1.7 μm (mean ± SEM) in the area centralis region. The thickness of the retinal nerve fiber layer (mean ± SEM) using a circular peripapillary retinal scan centered on the optic nerve was 46.7 ± 0.9 μm (temporal), 46.1 ± 0.9 μm (superior), 45.8 ± 0.9 μm (nasal), and 48.4 ± 1 μm (inferior). The baseline pupil diameter was 2.1 ± 0.05 mm in darkness, and 1.1 ± 0.05 and 0.56 ± 0.03 mm after stimulation with red (630 nm, luminance 200 kcd/m2) or blue (480 nm, luminance 200 kcd/m2) light illumination, respectively.
Conclusions Pattern electroretinography, cPLR and SD-OCT analysis are reproducible techniques, which can provide important information about retinal and optic nerve function and structure in mice.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Mohan, Kabhilan; Harper, Matthew M.; Kecova, Helga; Ye, Eun-Ah; Lazic, Tatjana; Sakaguchi, Donald S.; Kardon, Randy H.; Grozdanic, Sinisa D.; and CR: BioMed Vision Technologies, Neuroptics Inc., "Characterization of structure and function of the mouse retina using pattern electroretinography, pupil light reflex, and optical coherence tomography" (2012). Genetics, Development and Cell Biology Publications. 145.
https://lib.dr.iastate.edu/gdcb_las_pubs/145
Comments
This article is from Veterinary Ophthalmology 15 (2012): 94, doi: 10.1111/j.1463-5224.2012.01034.x.