Title
Patterns of Distribution of Oxygen-Binding Globins, Neuroglobin and Cytoglobin in Human Retina
Campus Units
Biochemistry, Biophysics and Molecular Biology, Genetics, Development and Cell Biology, Veterinary Clinical Sciences, Neuroscience
Document Type
Article
Publication Version
Published Version
Publication Date
2008
Journal or Book Title
Archives of Ophthalmology
Volume
126
Issue
11
First Page
1530
Last Page
1536
DOI
10.1001/archopht.126.11.1530
Abstract
Objective To determine the distribution of 2 intracellular oxygen-carrying molecules, neuroglobin (NGB) and cytoglobin (CYGB), in specific retinal cell types of human retinas.
Methods Specific antibodies against NGB and CYGB were used in immunohistochemical studies to examine their distribution patterns in human retinal sections. Double-labeling studies were performed with the anti-NGB and anti-CYGB antibodies along with antibodies against neuronal (microtubule-associated protein 2, class III β-tubulin [TUJ1], protein kinase C alpha, calretinin) and glial (vimentin, glial fibrillary acid protein) markers. Confocal microscopy was used to examine the retinal sections.
Results Immunohistochemical analysis of human retinal tissue showed NGB and CYGB immunoreactivity in the ganglion cell layer, inner nuclear layer, inner and outer plexiform layers, and retinal pigment epithelium. Neuroglobin immunoreactivity was also present in the outer nuclear layer and photoreceptor inner segments. Neuroglobin and CYGB were coexpressed in the neurons in the ganglion cell layer and inner nuclear layer but not within glial cells.
Conclusion Neuroglobin and CYGB are colocalized within human retinal neurons and retinal pigment epithelium but not within glial cells.
Clinical Relevance Our results suggest that NGB and CYGB may serve a neuroprotective role as scavengers of reactive oxygen species and therefore should be considered when developing therapeutic strategies for treatment of hypoxia-related ocular diseases.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Ostojic, Jelena; Grozdanic, Sinsia D.; Syed, Nasreen S.; Hargrove, Mark S.; Trent, James T. III; Kuehn, Markus H.; Kwon, Young H.; Kardon, Randy H.; and Sakaguchi, Donald S., "Patterns of Distribution of Oxygen-Binding Globins, Neuroglobin and Cytoglobin in Human Retina" (2008). Genetics, Development and Cell Biology Publications. 152.
https://lib.dr.iastate.edu/gdcb_las_pubs/152
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Cell and Developmental Biology Commons, Medical Sciences Commons, Tissues Commons
Comments
This article is from Archives of Ophthalmology 126 (2008): 1530, doi: 10.1001/archopht.126.11.1530.