Campus Units

Genetics, Development and Cell Biology, Statistics

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

8-15-2017

Journal or Book Title

Journal of Comparative Neurology

Volume

525

Issue

12

First Page

2735

Last Page

2781

DOI

10.1002/cne.24241

Abstract

The vertebrate retina is a specialized photosensitive tissue comprised of six neuronal and one glial cell types, each of which develops in prescribed proportions at overlapping timepoints from a common progenitor pool. While each of these cells has a specific function contributing to proper vision in the mature animal, their differential representation in the retina as well as the presence of distinctive cellular subtypes makes identifying the transcriptomic signatures that lead to each retinal cell’s fate determination and development challenging. We have analyzed transcriptomes from individual cells isolated from the chick retina throughout retinogenesis. While we focused our efforts on the retinal ganglion cells, our transcriptomes of developing chick cells also contained representation from multiple retinal cell types, including photoreceptors and interneurons at different stages of development. Most interesting was the identification of transcriptomes from individual mixed lineage progenitor cells in the chick as these cells offer a window into the cell fate decision-making process. Taken together, these datasets will enable us to uncover the most critical genes acting in the steps of cell fate determination and early differentiation of various retinal cell types.

Comments

This is the peer reviewed version of the following article: Laboissonniere, Lauren A., Gregory M. Martin, Jillian J. Goetz, Ran Bi, Brock Pope, Kallie Weinand, Laura Ellson et al. "Single cell transcriptome profiling of developing chick retinal cells." Journal of Comparative Neurology (2017) which has been published in final form at doi: 10.1002/cne.24241. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Copyright Owner

Wiley Periodicals, Inc.

Language

en

File Format

application/pdf

Available for download on Wednesday, August 15, 2018

Published Version

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