Campus Units

Genetics, Development and Cell Biology, Agronomy, Genetics and Genomics, Center for Plant Genomics

Document Type

Article

Publication Version

Published Version

Publication Date

8-2006

Journal or Book Title

Genetics

Volume

173

Issue

4

First Page

2211

Last Page

2226

DOI

10.1534/genetics.105.052712

Abstract

Tandemly arrayed duplicate genes are prevalent. The maize A1-b haplotype is a tandem duplication that consists of the components, a and b. The rate of meiotic unequal recombination at A1-b is ninefold higher when a homolog is present than when it is absent (i.e., hemizygote). When a sequence heterologous homolog is available, 94% of recombinants (264/281) are generated via recombination with the homolog rather than with the sister chromatid. In addition, 83% (220/264) of homolog recombination events involved a rather than b. These results indicate that: (1) the homolog is the preferred template for unequal recombination and (2) pairing of the duplicated segments with the homolog does not occur randomly but instead favors a particular configuration. The choice of recombination template (i.e., homolog vs. sister chromatid) affects the distribution of recombination breakpoints within a1. Rates of unequal recombination at A1-b are similar to the rate of recombination between nonduplicated a1 alleles. Unequal recombination is therefore common and is likely to be responsible for the generation of genetic variability, even within inbred lines.

Comments

This article is published as Yandeau-Nelson, Marna D., Yiji Xia, Jin Li, M. Gerald Neuffer, and Patrick S. Schnable. "Unequal sister chromatid and homolog recombination at a tandem duplication of the a1 locus in maize." Genetics 173, no. 4 (2006): 2211-2226. doi: 10.1534/genetics.105.052712.

Rights

Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.

Language

en

File Format

application/pdf

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