Campus Units

Genetics, Development and Cell Biology, Chemistry, Ames Laboratory, Plant Sciences Institute

Document Type

Article

Publication Version

Published Version

Publication Date

2015

Journal or Book Title

Scientific Reports

Volume

5

First Page

15694

DOI

10.1038/srep15694

Abstract

Super-resolution fluorescence microscopy has generated tremendous success in revealing detailed subcellular structures in animal cells. However, its application to plant cell biology remains extremely limited due to numerous technical challenges, including the generally high fluorescence background of plant cells and the presence of the cell wall. In the current study, stochastic optical reconstruction microscopy (STORM) imaging of intact Arabidopsis thaliana seedling roots with a spatial resolution of 20–40 nm was demonstrated. Using the super-resolution images, the spatial organization of cortical microtubules in different parts of a whole Arabidopsis root tip was analyzed quantitatively, and the results show the dramatic differences in the density and spatial organization of cortical microtubules in cells of different differentiation stages or types. The method developed can be applied to plant cell biological processes, including imaging of additional elements of the cytoskeleton, organelle substructure, and membrane domains.

Comments

This article is published as Dong, Bin, Xiaochen Yang, Shaobin Zhu, Diane C. Bassham, and Ning Fang. "Stochastic optical reconstruction microscopy imaging of microtubule arrays in intact Arabidopsis thaliana seedling roots." Scientific reports 5 (2015): 15694. doi: 10.1038/srep15694.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

Springer Nature Publishing AG

Language

en

File Format

application/pdf

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