Campus Units

Genetics, Development and Cell Biology

Document Type

Article

Publication Version

Published Version

Publication Date

12-5-2018

Journal or Book Title

Bio-protocol

Volume

8

Issue

23

First Page

e3101

DOI

10.21769/BioProtoc.3101

Abstract

Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol (DTT)-mediated root growth inhibition, analysis of splicing of the UPR-responsive transcription factor bZIP60 (basic Leucine Zipper Domain 60), and upregulation of relevant UPR genes. We provide here a quick and robust method to detect UPR signaling in Arabidopsis thaliana protoplasts. This assay can also be applied to other plant species for which protoplasts can be isolated.

Comments

This article is published Bao, Yan, and Diane C. Bassham. "Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling." Bio-protocol 8, no. 23 (2018) e3101. doi: 10.21769/BioProtoc.3101. Posted with permission.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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