Campus Units
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Genetics, Development and Cell Biology
Document Type
Article
Publication Version
Submitted Manuscript
Publication Date
11-27-2019
Journal or Book Title
bioRxiv
DOI
10.1101/857771
Abstract
Nectar is a primary reward mediating plant-animal mutualisms to improve plant fitness and reproductive success. In Gossypium hirsutum (cotton), four distinct trichomatic nectaries develop, one floral and three extrafloral. The secreted floral and extrafloral nectars serve different purposes, with the floral nectar attracting bees to promote pollination and the extrafloral nectar attracting predatory insects as a means of indirect resistance from herbivores. Cotton therefore provides an ideal system to contrast mechanisms of nectar production and nectar composition between floral and extrafloral nectaries. Here, we report the transcriptome, ultrastructure, and metabolite spatial distribution using mass spectrometric imaging of the four cotton nectary types throughout development. Additionally, the secreted nectar metabolomes were defined and were jointly composed of 197 analytes, 60 of which were identified. Integration of theses datasets support the coordination of merocrine-based and eccrine-based models of nectar synthesis. The nectary ultrastructure supports the merocrine-based model due to the abundance of rough endoplasmic reticulum positioned parallel to the cell walls and profusion of vesicles fusing to the plasma membranes. The eccrine-based model which consist of a progression from starch synthesis to starch degradation and to sucrose biosynthesis was supported by gene expression data. This demonstrates conservation of the eccrine-based model for the first time in both trichomatic and extrafloral nectaries. Lastly, nectary gene expression data provided evidence to support de novo synthesis of amino acids detected in the secreted nectars.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Copyright Owner
The Authors
Copyright Date
2019
Language
en
File Format
application/pdf
Recommended Citation
Chatt, Elizabeth C.; Mahalim, Siti-Nabilla; Mohd-Fadzil, Nur-Aziatull; Roy, Rahul; Klinkenberg, Peter M.; Horner, Harry T.; Hampton, Marshall; Carter, Clay J.; and Nikolau, Basil J., "Systems analyses of key metabolic modules of floral and extrafloral nectaries of cotton" (2019). Genetics, Development and Cell Biology Publications. 247.
https://lib.dr.iastate.edu/gdcb_las_pubs/247
Comments
This preprint is made available through bioRxiv at, doi: 10.1101/857771.